This standard is concerned with determination of nicotine in tobacco and tobacco products by gas chromatographic (GC) procedure using MTBE or n-hexane as a solvent. The method is applicable to raw tobacco as well as tobacco taken from finished products. The method is applicable for nicotine contents ranging at least from a mass fraction of 8 mg/g to 41 mg/g by dry weight. The method is applicable to any type of tobacco sample whose particle size has been reduced to totally pass through a 4 mm screen. Cut filler from cigarettes need not be reduced further in size.